MycoFog ® FAQs & Troubleshooting Guide
General Questions
1. What is MycoFog®?
MycoFog® is an advanced biodecontamination system that uses hydrogen peroxide (H2O2) fogging to eliminate microbial contamination from incubators, workstations, and other enclosed spaces.
2. What reagents can I use with MycoFog®?
- MycoFog® H2O2 reagents (our approved reagent for optimal performance):
| MycoFog® reagent, 4 bottles + 4 wicks. For incubators <200L | MFR-1Bx-K |
| MycoFog® reagent, 4 bottles + 4 wicks. For incubators >200L and <350L | MFR-2Bx-K |
| MycoFog® reagent, 2 bottles + 2 wicks. For incubators >350L and <500L | MFR-3Bx-K |
| MycoFog® reagent, 2 bottles + 2 wicks. For incubators >500L and <700L | MFR-4Bx-K |
| MycoFog® reagent, 2 bottles + 2 wicks. For incubators >700L and <1000L | MFR-5Bx-K |
| MycoFog® reagent, 2 bottles + 2 wicks. For incubators >1000L and <1200L | MFR-6Bx-K |
- Other compatible H2O2 solutions can be used (but please ensure proper concentration and purity).
Check out the link for MycoFog® reagents
3. How often should I perform a MycoFog® decontamination cycle?
For optimal contamination control, we recommend performing a MycoFog® cycle once per month per incubator.
SET UP & OPERATION
4. How do I prepare MycoFog® for a decontamination cycle?
Ensure your incubator is clean! The MycoFog® biodecontamination process complements cleaning but does not replace it. Please consult your incubator operating manual for the manufacturer’s cleaning protocol.
Ensure the incubator is empty (remove cultures, labware, and water pans).
Set the temperature to 45°C (or use the 37°C protocol for 4 hours).
Disable gas control (CO2 and humidity settings).
Place MycoFog® at the mid-level inside the incubator for optimal fog distribution.
Press the function button to start the cycle.
5. How long does the MycoFog® cycle take?
Standard Cycle: 180 minutes at 45°C
37°C Protocol: 4 hours for <200L chambers, 5 hours for larger chambers
6. Can I use MycoFog® in large incubators?
Yes! There are 2 instrument models of MycoFog®:
– Model MF-1B for incubators up to <500L internal volume
– Model MF-500 for incubators >500L but <1200L internal volume
MycoFog® reagents come in 6 different pack sizes, each formulation is designed for a size of Incubator, Glovebox, or Workstation.
Take care to order the correct MycoFog® Reagent pack for your instrument.
Table: Choose the right MycoFog® products
| Table to help Customers choose the right MycoFog® products | ||||
|---|---|---|---|---|
| MycoFog® | Incubator or Workstation to be decontaminated - Internal Vol (L) | MycoFog® Instrument needed | MycoFog® Reagent Kit Product code needed | MycoFog® Starter Kit needed |
| <200L | MF 1B | MF-1Bx | MF-SK-1Bx | |
| >200L <350L | MF-2Bx | MF-SK-2Bx | ||
| >350L <500L | MF-2Bx | MF-SK-3Bx | ||
| >500L <700L | MF-500 | MFR-4Bx-K | MF500-SK-4Bx | |
| >700L <1000L | MFR-5Bx-K | MF500-SK-5Bx | ||
| >1000L <1200L | MFR-6Bx-K | MF500-SK-6Bx | ||
7. What is the recommended relative humidity before starting a cycle?
MycoFog® works best when the incubator’s starting relative humidity is ambient.
The device achieves proper humidity during the cycle.
8. Can I use MycoFog® in an incubator that contains powered electronics?
Yes. MycoFog® has been tested with electronic devices such as the Etaluma Lumascope and the Sartorius Incucyte, which perform normally after repeated decontamination cycles.
TROUBLESHOOTING
9. My MycoFog® instrument is not producing enough spray. What should I do?
Check the Wick: Remove wick, ensure it is fully saturated, and reseat wick. Ensure wick holder is seated properly.
Check the Reagent: Ensure the correct amount is added and that only MycoFog® H2O2 reagent or an approved formulation is used.
Clean the Fogger: Use a cotton swab with vinegar to clean the nebulizer element.
Charge the Battery: Low power can reduce spray intensity — fully charge before use.
10. My MycoFog® stopped mid-cycle. How do I fix it?
Battery may be low — recharge the unit.
Restart the device by turning it off and on again.
11. The reagent is leaking — what should I check?
Ensure the reagent reservoir is properly sealed.
Confirm that the instrument is level and not tilted.
Do not overfill — use only the recommended reagent volume.
Device Performance & Maintenance
12. How long does the battery last?
A fully charged battery will perform approximately 10 cycles.
We recommend fully charging the instrument before each use if repeated uses are done on different days.
13. Can MycoFog® operate while charging?
Yes, MycoFog® can function while connected to a power source.
14. How do I clean my MycoFog® instrument?
After each use, rinse the reagent reservoir with distilled water.
If fogging volume decreases, clean the nebulizer disk with vinegar and a cotton swab.
Use a fresh wick for each cycle.
15. What quality control checks are performed before shipping?
Each MycoFog® instrument is tested to ensure:
– Proper flow rate within the acceptable range
– Residual reagent volume is below the maximum allowable level
Validation & Compliance
16. Can MycoFog® be used in a GMP environment?
Yes! Our solvent-resistant instrument labels allow for alcohol wipe-downs before GMP suite entry.
17. How do I validate MycoFog®’s effectiveness?
Use an approved Biological Indicator (BI’s) to confirm a reduction in microbial contamination.
Ordering & Support
18. Where can I purchase MycoFog® and MycoFog® reagents?
For US/Canada, visit our website at https://mycofog.com and click “Get MycoFog®”.
For international customers, please visit https://mycofog.com and contact your authorized Partner.
19. Need Help?
For troubleshooting, orders, or technical support, contact us at:
[email protected]
MycoFog ® FAQs & Troubleshooting Guide
General Questions
1. What is MycoFog®?
MycoFog® is an advanced biodecontamination system that uses hydrogen peroxide (H2O2) fogging to eliminate microbial contamination from incubators, workstations, and other enclosed spaces.
2. What reagents can I use with MycoFog®?
- MycoFog® H2O2 reagents (our approved reagent for optimal performance):
| MycoFog® reagent, 4 bottles + 4 wicks. For incubators <200L | MFR-1Bx-K |
| MycoFog® reagent, 4 bottles + 4 wicks. For incubators >200L and <350L | MFR-2Bx-K |
| MycoFog® reagent, 2 bottles + 2 wicks. For incubators >350L and <500L | MFR-3Bx-K |
| MycoFog® reagent, 2 bottles + 2 wicks. For incubators >500L and <700L | MFR-4Bx-K |
| MycoFog® reagent, 2 bottles + 2 wicks. For incubators >700L and <1000L | MFR-5Bx-K |
| MycoFog® reagent, 2 bottles + 2 wicks. For incubators >1000L and <1200L | MFR-6Bx-K |
- Other compatible H2O2 solutions can be used (but please ensure proper concentration and purity).
Check out the link for MycoFog® reagents
3. How often should I perform a MycoFog® decontamination cycle?
For optimal contamination control, we recommend performing a MycoFog® cycle once per month per incubator.
SET UP & OPERATION
4. How do I prepare MycoFog® for a decontamination cycle?
Ensure your incubator is clean! The MycoFog® biodecontamination process complements cleaning but does not replace it. Please consult your incubator operating manual for the manufacturer’s cleaning protocol.
Ensure the incubator is empty (remove cultures, labware, and water pans).
Set the temperature to 45°C (or use the 37°C protocol for 4 hours).
Disable gas control (CO2 and humidity settings).
Place MycoFog® at the mid-level inside the incubator for optimal fog distribution.
Press the function button to start the cycle.
5. How long does the MycoFog® cycle take?
Standard Cycle: 180 minutes at 45°C
37°C Protocol: 4 hours for <200L chambers, 5 hours for larger chambers
6. Can I use MycoFog® in large incubators?
Yes! There are 2 instrument models of MycoFog®:
– Model MF-1B for incubators up to <500L internal volume
– Model MF-500 for incubators >500L but <1200L internal volume
MycoFog® reagents come in 6 different pack sizes, each formulation is designed for a size of Incubator, Glovebox, or Workstation.
Take care to order the correct MycoFog® Reagent pack for your instrument.
Ensure your incubator is clean! The MycoFog® biodecontamination process complements cleaning but does not replace it. Please consult your incubator operating manual for the manufacturer’s cleaning protocol.
Ensure the incubator is empty (remove cultures, labware, and water pans).
Set the temperature to 45°C (or use the 37°C protocol for 4 hours).
Disable gas control (CO2 and humidity settings).
Place MycoFog® at the mid-level inside the incubator for optimal fog distribution.
Press the function button to start the cycle.
5. How long does the MycoFog® cycle take?
Standard Cycle: 180 minutes at 45°C
37°C Protocol: 4 hours for <200L chambers, 5 hours for larger chambers
6. Can I use MycoFog® in large incubators?
Yes! There are 2 instrument models of MycoFog®:
– Model MF-1B for incubators up to <500L internal volume
– Model MF-500 for incubators >500L but <1200L internal volume
MycoFog® reagents come in 6 different pack sizes, each formulation is designed for a size of Incubator, Glovebox, or Workstation.
Take care to order the correct MycoFog® Reagent pack for your instrument.
| Table to help Customers choose the right MycoFog® products | ||||
|---|---|---|---|---|
| MycoFog® | Incubator or Workstation to be decontaminated – Internal Vol (L) | MycoFog® Instrument needed | MycoFog® Reagent Kit Product code needed | MycoFog® Starter Kit needed |
| <200L | MF 1B | MF-1Bx | MF-SK-1Bx | |
| >200L <350L | MF-2Bx | MF-SK-2Bx | ||
| >350L <500L | MF-2Bx | MF-SK-3Bx | ||
| >500L <700L | MF-500 | MFR-4Bx-K | MF500-SK-4Bx | |
| >700L <1000L | MFR-5Bx-K | MF500-SK-5Bx | ||
| >1000L <1200L | MFR-6Bx-K | MF500-SK-6Bx | ||
7. What is the recommended relative humidity before starting a cycle?
MycoFog® works best when the incubator’s starting relative humidity is ambient.
The device achieves proper humidity during the cycle.
8. Can I use MycoFog® in an incubator that contains powered electronics?
Yes. MycoFog® has been tested with electronic devices such as the Etaluma Lumascope and the Sartorius Incucyte, which perform normally after repeated decontamination cycles.
MycoFog® works best when the incubator’s starting relative humidity is ambient.
The device achieves proper humidity during the cycle.
8. Can I use MycoFog® in an incubator that contains powered electronics?
Yes. MycoFog® has been tested with electronic devices such as the Etaluma Lumascope and the Sartorius Incucyte, which perform normally after repeated decontamination cycles.
TROUBLESHOOTING
9. My MycoFog® instrument is not producing enough spray. What should I do?
Check the Wick: Remove wick, ensure it is fully saturated, and reseat wick. Ensure wick holder is seated properly.
Check the Reagent: Ensure the correct amount is added and that only MycoFog® H2O2 reagent or an approved formulation is used.
Clean the Fogger: Use a cotton swab with vinegar to clean the nebulizer element.
Charge the Battery: Low power can reduce spray intensity — fully charge before use.
10. My MycoFog® stopped mid-cycle. How do I fix it?
Battery may be low — recharge the unit.
Restart the device by turning it off and on again.
11. The reagent is leaking — what should I check?
Ensure the reagent reservoir is properly sealed.
Confirm that the instrument is level and not tilted.
Do not overfill — use only the recommended reagent volume.
Check the Wick: Remove wick, ensure it is fully saturated, and reseat wick. Ensure wick holder is seated properly.
Check the Reagent: Ensure the correct amount is added and that only MycoFog® H2O2 reagent or an approved formulation is used.
Clean the Fogger: Use a cotton swab with vinegar to clean the nebulizer element.
Charge the Battery: Low power can reduce spray intensity — fully charge before use.
10. My MycoFog® stopped mid-cycle. How do I fix it?
Battery may be low — recharge the unit.
Restart the device by turning it off and on again.
11. The reagent is leaking — what should I check?
Ensure the reagent reservoir is properly sealed.
Confirm that the instrument is level and not tilted.
Do not overfill — use only the recommended reagent volume.
Device Performance & Maintenance
12. How long does the battery last?
A fully charged battery will perform approximately 10 cycles.
We recommend fully charging the instrument before each use if repeated uses are done on different days.
13. Can MycoFog® operate while charging?
Yes, MycoFog® can function while connected to a power source.
14. How do I clean my MycoFog® instrument?
After each use, rinse the reagent reservoir with distilled water.
If fogging volume decreases, clean the nebulizer disk with vinegar and a cotton swab.
Use a fresh wick for each cycle.
15. What quality control checks are performed before shipping?
Each MycoFog® instrument is tested to ensure:
– Proper flow rate within the acceptable range
– Residual reagent volume is below the maximum allowable level
A fully charged battery will perform approximately 10 cycles.
We recommend fully charging the instrument before each use if repeated uses are done on different days.
13. Can MycoFog® operate while charging?
Yes, MycoFog® can function while connected to a power source.
14. How do I clean my MycoFog® instrument?
After each use, rinse the reagent reservoir with distilled water.
If fogging volume decreases, clean the nebulizer disk with vinegar and a cotton swab.
Use a fresh wick for each cycle.
15. What quality control checks are performed before shipping?
Each MycoFog® instrument is tested to ensure:
– Proper flow rate within the acceptable range
– Residual reagent volume is below the maximum allowable level
Validation & Compliance
16. Can MycoFog® be used in a GMP environment?
Yes! Our solvent-resistant instrument labels allow for alcohol wipe-downs before GMP suite entry.
17. How do I validate MycoFog®’s effectiveness?
Use an approved Biological Indicator (BI’s) to confirm a reduction in microbial contamination.
Yes! Our solvent-resistant instrument labels allow for alcohol wipe-downs before GMP suite entry.
17. How do I validate MycoFog®’s effectiveness?
Use an approved Biological Indicator (BI’s) to confirm a reduction in microbial contamination.
Ordering & Support
18. Where can I purchase MycoFog® and MycoFog® reagents?
For US/Canada, visit our website at https://mycofog.com and click “Get MycoFog®”.
For international customers, please visit https://mycofog.com and contact your authorized Partner.
19. Need Help?
For troubleshooting, orders, or technical support, contact us at:
[email protected]
For US/Canada, visit our website at https://mycofog.com and click “Get MycoFog®”.
For international customers, please visit https://mycofog.com and contact your authorized Partner.
19. Need Help?
For troubleshooting, orders, or technical support, contact us at:
[email protected]
MycoFog ® FAQs & Troubleshooting Guide
General Questions
1. What is MycoFog®?
MycoFog® is an advanced biodecontamination system that uses hydrogen peroxide (H2O2) fogging to eliminate microbial contamination from incubators, workstations, and other enclosed spaces.
2. What reagents can I use with MycoFog®?
Check out the link for MycoFog® reagents
3. How often should I perform a MycoFog® decontamination cycle?
For optimal contamination control, we recommend performing a MycoFog® cycle once per month per incubator.
MycoFog® is an advanced biodecontamination system that uses hydrogen peroxide (H2O2) fogging to eliminate microbial contamination from incubators, workstations, and other enclosed spaces.
2. What reagents can I use with MycoFog®?
- MycoFog® H2O2 reagents (our approved reagent for optimal performance):
| MycoFog® reagent, 4 bottles + 4 wicks. For incubators <200L | MFR-1Bx-K |
| MycoFog® reagent, 4 bottles + 4 wicks. For incubators >200L and <350L | MFR-2Bx-K |
| MycoFog® reagent, 2 bottles + 2 wicks. For incubators >350L and <500L | MFR-3Bx-K |
| MycoFog® reagent, 2 bottles + 2 wicks. For incubators >500L and <700L | MFR-4Bx-K |
| MycoFog® reagent, 2 bottles + 2 wicks. For incubators >700L and <1000L | MFR-5Bx-K |
| MycoFog® reagent, 2 bottles + 2 wicks. For incubators >1000L and <1200L | MFR-6Bx-K |
- Other compatible H2O2 solutions can be used (but please ensure proper concentration and purity).
Check out the link for MycoFog® reagents
3. How often should I perform a MycoFog® decontamination cycle?
For optimal contamination control, we recommend performing a MycoFog® cycle once per month per incubator.
SET UP & OPERATION
4. How do I prepare MycoFog® for a decontamination cycle?
Ensure your incubator is clean! The MycoFog® biodecontamination process complements cleaning but does not replace it. Please consult your incubator operating manual for the manufacturer’s cleaning protocol.
Ensure the incubator is empty (remove cultures, labware, and water pans).
Set the temperature to 45°C (or use the 37°C protocol for 4 hours).
Disable gas control (CO2 and humidity settings).
Place MycoFog® at the mid-level inside the incubator for optimal fog distribution.
Press the function button to start the cycle.
5. How long does the MycoFog® cycle take?
Standard Cycle: 180 minutes at 45°C
37°C Protocol: 4 hours for <200L chambers, 5 hours for larger chambers
6. Can I use MycoFog® in large incubators?
Yes! There are 2 instrument models of MycoFog®:
– Model MF-1B for incubators up to <500L internal volume
– Model MF-500 for incubators >500L but <1200L internal volume
MycoFog® reagents come in 6 different pack sizes, each formulation is designed for a size of Incubator, Glovebox, or Workstation.
Take care to order the correct MycoFog® Reagent pack for your instrument.
Ensure your incubator is clean! The MycoFog® biodecontamination process complements cleaning but does not replace it. Please consult your incubator operating manual for the manufacturer’s cleaning protocol.
Ensure the incubator is empty (remove cultures, labware, and water pans).
Set the temperature to 45°C (or use the 37°C protocol for 4 hours).
Disable gas control (CO2 and humidity settings).
Place MycoFog® at the mid-level inside the incubator for optimal fog distribution.
Press the function button to start the cycle.
5. How long does the MycoFog® cycle take?
Standard Cycle: 180 minutes at 45°C
37°C Protocol: 4 hours for <200L chambers, 5 hours for larger chambers
6. Can I use MycoFog® in large incubators?
Yes! There are 2 instrument models of MycoFog®:
– Model MF-1B for incubators up to <500L internal volume
– Model MF-500 for incubators >500L but <1200L internal volume
MycoFog® reagents come in 6 different pack sizes, each formulation is designed for a size of Incubator, Glovebox, or Workstation.
Take care to order the correct MycoFog® Reagent pack for your instrument.
| Table to help Customers choose the right MycoFog® products | ||||
|---|---|---|---|---|
| MycoFog® | Incubator or Workstation to be decontaminated – Internal Vol (L) | MycoFog® Instrument needed | MycoFog® Reagent Kit Product code needed | MycoFog® Starter Kit needed |
| <200L | MF 1B | MF-1Bx | MF-SK-1Bx | |
| >200L <350L | MF-2Bx | MF-SK-2Bx | ||
| >350L <500L | MF-2Bx | MF-SK-3Bx | ||
| >500L <700L | MF-500 | MFR-4Bx-K | MF500-SK-4Bx | |
| >700L <1000L | MFR-5Bx-K | MF500-SK-5Bx | ||
| >1000L <1200L | MFR-6Bx-K | MF500-SK-6Bx | ||
7. What is the recommended relative humidity before starting a cycle?
MycoFog® works best when the incubator’s starting relative humidity is ambient.
The device achieves proper humidity during the cycle.
8. Can I use MycoFog® in an incubator that contains powered electronics?
Yes. MycoFog® has been tested with electronic devices such as the Etaluma Lumascope and the Sartorius Incucyte, which perform normally after repeated decontamination cycles.
MycoFog® works best when the incubator’s starting relative humidity is ambient.
The device achieves proper humidity during the cycle.
8. Can I use MycoFog® in an incubator that contains powered electronics?
Yes. MycoFog® has been tested with electronic devices such as the Etaluma Lumascope and the Sartorius Incucyte, which perform normally after repeated decontamination cycles.
TROUBLESHOOTING
9. My MycoFog® instrument is not producing enough spray. What should I do?
Check the Wick: Remove wick, ensure it is fully saturated, and reseat wick. Ensure wick holder is seated properly.
Check the Reagent: Ensure the correct amount is added and that only MycoFog® H2O2 reagent or an approved formulation is used.
Clean the Fogger: Use a cotton swab with vinegar to clean the nebulizer element.
Charge the Battery: Low power can reduce spray intensity — fully charge before use.
10. My MycoFog® stopped mid-cycle. How do I fix it?
Battery may be low — recharge the unit.
Restart the device by turning it off and on again.
11. The reagent is leaking — what should I check?
Ensure the reagent reservoir is properly sealed.
Confirm that the instrument is level and not tilted.
Do not overfill — use only the recommended reagent volume.
Check the Wick: Remove wick, ensure it is fully saturated, and reseat wick. Ensure wick holder is seated properly.
Check the Reagent: Ensure the correct amount is added and that only MycoFog® H2O2 reagent or an approved formulation is used.
Clean the Fogger: Use a cotton swab with vinegar to clean the nebulizer element.
Charge the Battery: Low power can reduce spray intensity — fully charge before use.
10. My MycoFog® stopped mid-cycle. How do I fix it?
Battery may be low — recharge the unit.
Restart the device by turning it off and on again.
11. The reagent is leaking — what should I check?
Ensure the reagent reservoir is properly sealed.
Confirm that the instrument is level and not tilted.
Do not overfill — use only the recommended reagent volume.
Device Performance & Maintenance
12. How long does the battery last?
A fully charged battery will perform approximately 10 cycles.
We recommend fully charging the instrument before each use if repeated uses are done on different days.
13. Can MycoFog® operate while charging?
Yes, MycoFog® can function while connected to a power source.
14. How do I clean my MycoFog® instrument?
After each use, rinse the reagent reservoir with distilled water.
If fogging volume decreases, clean the nebulizer disk with vinegar and a cotton swab.
Use a fresh wick for each cycle.
15. What quality control checks are performed before shipping?
Each MycoFog® instrument is tested to ensure:
– Proper flow rate within the acceptable range
– Residual reagent volume is below the maximum allowable level
A fully charged battery will perform approximately 10 cycles.
We recommend fully charging the instrument before each use if repeated uses are done on different days.
13. Can MycoFog® operate while charging?
Yes, MycoFog® can function while connected to a power source.
14. How do I clean my MycoFog® instrument?
After each use, rinse the reagent reservoir with distilled water.
If fogging volume decreases, clean the nebulizer disk with vinegar and a cotton swab.
Use a fresh wick for each cycle.
15. What quality control checks are performed before shipping?
Each MycoFog® instrument is tested to ensure:
– Proper flow rate within the acceptable range
– Residual reagent volume is below the maximum allowable level
Validation & Compliance
16. Can MycoFog® be used in a GMP environment?
Yes! Our solvent-resistant instrument labels allow for alcohol wipe-downs before GMP suite entry.
17. How do I validate MycoFog®’s effectiveness?
Use an approved Biological Indicator (BI’s) to confirm a reduction in microbial contamination.
Yes! Our solvent-resistant instrument labels allow for alcohol wipe-downs before GMP suite entry.
17. How do I validate MycoFog®’s effectiveness?
Use an approved Biological Indicator (BI’s) to confirm a reduction in microbial contamination.
Ordering & Support
18. Where can I purchase MycoFog® and MycoFog® reagents?
For US/Canada, visit our website at https://mycofog.com and click “Get MycoFog®”.
For international customers, please visit https://mycofog.com and contact your authorized Partner.
19. Need Help?
For troubleshooting, orders, or technical support, contact us at:
[email protected]
For US/Canada, visit our website at https://mycofog.com and click “Get MycoFog®”.
For international customers, please visit https://mycofog.com and contact your authorized Partner.
19. Need Help?
For troubleshooting, orders, or technical support, contact us at:
[email protected]
validation
The definitive validation of disinfection processes is the use of spores of the bacteria Geobacillus stearothermophilus. These spores are extremely hardy and are used to validate biodecontamination and disinfection processes in number of applications.
7.8 BIO tests the MycoFog® biodecontamination process by placing the biological indicator (BI) (from Mesa Labs or Terragene) which are stainless steel discs impregnated with at least 1 x 106 Geobacillus stearothermophilus (G.s.) spores, into the incubator. The device is sealed so that it is required that the sterilant (nebulized hydrogen peroxide, nHP) penetrate a membrane to come into contact with the spores, killing them.
7.8 BIO tests the MycoFog® biodecontamination process by placing the biological indicator (BI) (from Mesa Labs or Terragene) which are stainless steel discs impregnated with at least 1 x 106 Geobacillus stearothermophilus (G.s.) spores, into the incubator. The device is sealed so that it is required that the sterilant (nebulized hydrogen peroxide, nHP) penetrate a membrane to come into contact with the spores, killing them.
Locations in the incubator where the G.s. biological indicator devices (BI’s) are placed for a validation run.
The BI’s are placed in the appropriate position in the incubator and exposed to a full biodecontamination cycle, processed, and then incubated for 7 days.
Results from one validation run of Geobacillus stearothermophilus test devices described above. The control device is outside of the incubator chamber while the test devices are exposed to a complete biodecontamination cycle in the incubator. The media color changes to yellow when the spores grow, negative growth is indicated by the purple color. These results indicate successful biodecontamination of the incubator.
